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Hepatotoxin Production Kinetics of the Cyanobacterium Microcystis aeruginosa PCC 7820, as Determined by HPLC−Mass Spectrometry and Protein Phosphatase Bioassay
C. Robillot, J. Vinh, S. Puiseux‐Dao, M. Hennion
Freshwater cyanobacteria (blue-green algae) can produce numerous potent toxins and represent an increasing environmental hazard. The microcystin content (cyclic heptapeptidic toxins) of the hepatotoxic cyanobacterium Microcystis aeruginosa PCC 7820 …
Freshwater cyanobacteria (blue-green algae) can produce numerous potent toxins and represent an increasing environmental hazard. The microcystin content (cyclic heptapeptidic toxins) of the hepatotoxic cyanobacterium Microcystis aeruginosa PCC 7820 was investigated. Ten microcystins were identified using high performance liquid microchromatography (micro HPLC) coupled to either a ultraviolet (UV) diode-array detector (DAD) or an electrospray ionization (ESI) mass spectrometer. Three new variants were identified: desmethylated microcystin LW (mcyst-dMeLW), desmethylated microcystin LF (mcyst-dMeLF), and microcystin LL (mcyst-LL) by Collision-Induced Dissociation/Post-Source Decay Matrix-assisted Laser Desorption/Ionization−Time-of-Flight mass spectrometry (CID/PSD MALDI-TOF MS). The concentration of intracellular microcystins reached 2−8 mg/g of dried cells, with a microcystin LR (mcyst-LR) equivalent of 1−5 mg/g as estimated by protein phosphatase 2A (PP2A) inhibition assay. Toxin production can be corre...
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6 | 2000 |
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