| Title | Claps | Level | Year | L/Y |
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Effects of Prolonged Exposure in Vitro to Interferon-γ and Tumour Necrosis Factor-α on Nitric Oxide and Insulin Production of Rat Pancreatic Islets
J. Sternesjö, K. Bendtzen, S. Sandler
It has been postulated that cytokines may mediate the β-cell destructive process causing insulin-dependent diabetes mellitus. The aim of this investigation was to study cytokine effects on pancreatic islet functions in vitro. For this purpose 5–7 da…
It has been postulated that cytokines may mediate the β-cell destructive process causing insulin-dependent diabetes mellitus. The aim of this investigation was to study cytokine effects on pancreatic islet functions in vitro. For this purpose 5–7 days precultured (medium RPMI 1640+ 10% fetal calf serum) rat pancreatic islets were exposed for another 48 h to either culture medium alone or with addition of rat interferon-γ (IFN-γ; 1000 U/ml), or human tumor necrosis factor-α (TNF-α; 1000 U/ml) or a combination of the cytokines. After the culture period the islets were subjected to short-term experiments in the absence of cytokines. Neither the DNA nor the insulin content of the islets were affected by the cytokines alone or by the combination. The combination IFN-γ + TNF-α caused a 5-fold increase in the medium nitrite accumulation, indicating induction of nitric oxide formation. It was found that IFN-γ reduced medium insulin accumulation and basal insulin secretion at 1.7 mM glucose, without affecting the ...
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4 | 1995 |
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