| Title | Claps | Level | Year | L/Y |
|---|---|---|---|---|
|
Empirical Analysis of Transcriptional Activity in the Arabidopsis Genome
70 auth. Kayoko Yamada, Jun-Man Lim, Joseph M. Dale, Huaming Chen, P. Shinn, C. Palm, Audrey M. Southwick, Hank Wu, Christopher J. Kim, M. Nguyen, Paul K. Pham, Rosa Cheuk, George Karlin-Newmann, Shirley X. Liu, Bao Lam, ...
Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis. Sequen…
Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis. Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density oligonucleotide arrays spanning the entire genome allowed the accurate annotation of thousands of gene structures. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of ∼30% of the Arabidopsis ORFeome as a resource for global functional experimentation of the plant proteome.
Published in
Science
|
117
|
9 | 2003 |
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